Self‐Immolative Nanobody‐Cysteine Residue Modification for Controlled Immunodrug Delivery
نویسندگان
چکیده
Applications of antibody-drug conjugates are rapidly growing, however, arduous fabrication antibodies and impairment highly potent drugs by covalent fixation to the protein is urging for alternatives these conventional strategies. Here, we demonstrate a procedure on genetically engineered single domain antibodies, so-called nanobodies, their site-specific reversible bioconjugation using self-immolative linkers (SILs). Straight-forward fluorescent labelling at C-terminal cysteine could be reversed under reductive conditions due its disulfide-containing SIL. Flow cytometry microscopy images were able cellular uptake confirm integrity nanobodies’ biological affinity notwithstanding being modified. Following this strategy, small molecular immunomodulator installed stimulatory effect level was boosted in vitro compared non-degradable alternatives. Furthermore, protocol extended further therapeutically relevant representatives underlining versatility reductive-responsive broad field applications. This article protected copyright. All rights reserved
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ژورنال
عنوان ژورنال: Advanced therapeutics
سال: 2023
ISSN: ['2366-3987']
DOI: https://doi.org/10.1002/adtp.202300076